Studies on Streptomyces phage II. Host range and species differentiation.

Plaque morphology of certain streptomycete phages.

Phage host range is a useful marker in epidemiological and genetic studies of phages. Conversely, phages can often discriminate among organisms not separable serologically, thus constituting the basis of phage typing. Plaque morphology may provide identification of both host and phage still more precisely than host range or phage sensitivity. For purposes of such identification it would be valu...

Sequence-Based Differentiation of Strains in the Streptomyces cyaneus Species-Group

In an extensive numerical phenetic survey, a numberof blue, red and gray spored streptomycete strainswere grouped together as Streptomyces cyaneusspecies-group, a taxon which encompasses strainsknown to produce antitumor antibiotics, notably anthracyclines.In the present investigation these and relatedstreptomycetes were the subject of morphological and...

Studies on Streptomyces phage. I. Growth characteristics of the Streptomyces griseus host-phage system.

Within recent years, phages capable of infecting various Streptomyces species have been reported (Saudek and Colingsworth, 1947; Reilly et al., 1947; Koerber et al., 1950; Perlman et al., 1951; and Alexander and McCoy, 1956). Hoehn (1949) and subsequently Gilmour and Buthala (1950) demonstrated that streptomyces phage is indigenous to soil, the natural habitat of the host. The streptomyces host...

Isolation and characterization of a phage active against streptomyces griseus

A novel Streptomyces spp. integration vector derived from the S. venezuelae phage, SV1

BACKGROUND Integrating vectors based on the int/attP loci of temperate phages are convenient and used widely, particularly for cloning genes in Streptomyces spp. RESULTS We have constructed and tested a novel integrating vector based on g27, encoding integrase, and attP site from the phage, SV1. This plasmid, pBF3 integrates efficiently in S. coelicolor and S. lividans but surprisingly fails ...